circFOXK2靶向miR-4677-3p促进口腔鳞癌细胞增殖、侵袭和迁移

龚瑶; 李梦园; 章茜

doi:10.13898/j.cnki.issn.1000-2200.2024.02.001

circFOXK2靶向miR-4677-3p促进口腔鳞癌细胞增殖、侵袭和迁移

circFOXK2 targets miR-4677-3p to promote the proliferation, invasion and migration of oral squamous cell carcinoma cells

摘要

摘要:
目的探讨circFOXK2靶向miR-4677-3p对口腔鳞癌(OSCC)细胞恶性行为的影响。
方法RT-qPCR检测circFOXK2和miR-4677-3p在OSCC组织和癌旁组织的表达。分别将si-circFOXK2、si-NC、miR-NC、miR-4677-3p模拟物、pcDNA、pcDNA-circFOXK2、si-circFOXK2+anti-miR-NC、si-circFOXK2+anti-miR-4677-3p转染OSCC细胞HSC3。通过划痕愈合实验、CCK-8法、Transwell实验、集落形成实验检测circFOXK2和miR-4677-3p表达对HSC3细胞恶行行为的影响。circFOXK2和miR-4677-3p的靶向关系通过双荧光素酶法确定。
结果OSCC组织中circFOXK2表达水平显著高于癌旁组织(P < 0.01)，miR-4677-3p表达水平显著低于癌旁组织(P < 0.01)。抑制circFOXK2表达后HSC3细胞吸光度(OD)值、划痕愈合率、克隆形成数、侵袭数显著降低(P < 0.01)，miR-4677-3p表达水平显著升高(P < 0.01)。过表达miR-4677-3p后HSC3细胞OD值、划痕愈合率、克隆形成数、侵袭数显著降低(P < 0.01)。miR-4677-3p是circFOXK2的靶基因。下调miR-4677-3p显著减弱抑制circFOXK2表达对HSC3细胞OD值、划痕愈合率、克隆形成数、侵袭数的影响(P < 0.01)。
结论抑制circFOXK2可通过促进miR-4677-3p表达来抑制OSCC细胞增殖、迁移和侵袭。

Abstract:
ObjectiveTo study the effect of circFOXK2 targeting miR-4677-3p on the malignant behavior of oral squamous cell carcinoma (OSCC) cells.
MethodsThe expression of circFOXK2 and miR-4677-3p in OSCC tissues and adjacent tissues was calculated by RT-qPCR.Si-circFOXK2, si-NC, miR-NC, miR-4677-3p mimic, pcDNA, pcDNA-circFOXK2, si-circFOXK2+anti-miR-NC, and si-circFOXK2+anti-miR-4677-3p were respectively transfected into OSCC cells HSC3.The effects of circFOXK2 and miR-4677-3p expression on the malignant behavior of HSC3 cells were detected by scratch healing, CCK-8, Transwell and colony formation assays.The targeting relationship between circFOXK2 and miR-4677-3p was determined by dual luciferase method.
ResultsThe expression level of circFOXK2 in OSCC tissue was significantly higher than that in adjacent tissues (P < 0.01), while the expression level of miR-4677-3p was significantly lower than that in adjacent tissues (P < 0.01).After inhibiting the expression of circFOXK2, the HSC3 cell optical density (OD) value, scratch healing rate, clone formation number and invasion number were significantly reduced (P < 0.01), and the expression level of miR-4677-3p was significantly increased (P < 0.01).After overexpression of miR-4677-3p, the HSC3 cell OD value, scratch healing rate, clone formation number and invasion number were significantly reduced (P < 0.01).MiR-4677-3p was a target gene of circFOXK2.MiR-4677-3p down-regulation significantly reduced the effect of inhibiting circFOXK2 on cell OD value, scratch healing rate, clone formation number, and number of invasion of HSC3 cells (P < 0.01).
ConclusionsInhibition of circFOXK2 can suppress the proliferation, migration and invasion of OSCC cells by promoting miR-4677-3p expression.

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